in vitro azadirachtin production by hairy root cultivation
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in vitro azadirachtin production by hairy root cultivation .

In vitro azadirachtin production by hairy root cultivation ...

Azadirachtin, a well-known biopesticide is a secondary metabolite conventionally extracted from the seeds of Azadirachta indica. The present study involved in vitro azadirachtin production by developing hairy roots of A. indica via Agrobacterium rhizogenes-mediated transformation of A. indica explants.

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In Vitro Azadirachtin Production by Hairy Root Cultivation ...

2011-11-15  Azadirachtin, a well-known biopesticide is a secondary metabolite conventionally extracted from the seeds of Azadirachta indica. The present study involved in vitro azadirachtin production by developing hairy roots of A. indica via Agrobacterium rhizogenes-mediated transformation of A. indica explants. Liquid culture of hairy roots was established in shake flask to study the kinetics of growth ...

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In vitro azadirachtin production by hairy root cultivation ...

2011-11-15  Liquid culture of hairy roots was established in shake flask to study the kinetics of growth and azadirachtin production. A biomass production of 13.3 g/L dry weight (specific growth rate of 0.7 day(-1)) was obtained after 25 days of cultivation period with an azadirachtin yield of 3.3 mg/g root

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In Vitro Azadirachtin Production by Hairy Root Cultivation ...

Hairy root cultivation could be one of the potent in vitro alternative production techniques for artemisinin as it has an inherent advantage of better biochemical stability and less doubling time ...

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Production of the Biopesticide Azadirachtin by Hairy Root ...

2013-8-17  Batch cultivation of Azadirachta indica hairy roots was carried out in different liquid-phase bioreactor configurations (stirred-tank, bubble column, bubble column with polypropylene basket, and polyurethane foam disc as root supports) to investigate possible scale-up of the A. indica hairy root culture for in vitro production of the biopesticide azadirachtin.

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Production of the biopesticide azadirachtin by hairy root ...

Batch cultivation of Azadirachta indica hairy roots was carried out in different liquid-phase bioreactor configurations (stirred-tank, bubble column, bubble column with polypropylene basket, and polyurethane foam disc as root supports) to investigate possible scale-up of the A. indica hairy root culture for in vitro production of the biopesticide azadirachtin.

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Effect of elicitors and precursors on azadirachtin ...

The present study involved strategies for enhancement in in vitro azadirachtin (commercially used biopesticide) production by hairy root cultivation of Azadirachta indica. Improvement in the azadirachtin production via triggering its biosynthetic pathway in plant cells was carried out by the exogeno

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Production of the Biopesticide Azadirachtin by Hairy Root ...

Request PDF Production of the Biopesticide Azadirachtin by Hairy Root Cultivation of Azadirachta indica in Liquid-Phase Bioreactors Batch cultivation of Azadirachta indica hairy roots was ...

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Enhanced production of azadirachtin by hairy root cultures ...

2007-2-1  Hairy roots were cultured in 70 ml of liquid media in 250 ml Erlenmeyer flasks and kept in gyratory shaker at 60 rpm.When three different media like ON, B5 and MS with 3% sucrose were tested, it was found that ON was the best medium for the growth of hairy root cultures and azadirachtin production followed by B5 and MS, respectively ().The roots grown in ON medium produced 0.0166%

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Soybean hairy roots produced in vitro by Agrobacterium ...

2018-4-1  After infection and co-cultivation, hairy roots were produced in induction culture medium after 10–12 days. Using this method, 90%–99% of the infected explants of five different cultivars produced hairy roots within one month. Observations using reporter constructs showed that 30%–60% of the hairy roots induced were transformed.

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Azadirachtin production by hairy root cultivation of ...

2012-5-13  This included cultivation azadirachtin production was highest on 15th day of the growth cycle (2.96 mg/(l day)) as a result combined addition of a fast-growing, high-yielding hairy root line of A. indica under optimum cultivation conditions in the selected bio- of the elicitor and precursor on 15th day could possibly enhance the azadirachtin ...

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Production of the Biopesticide Azadirachtin by Hairy Root ...

Request PDF Production of the Biopesticide Azadirachtin by Hairy Root Cultivation of Azadirachta indica in Liquid-Phase Bioreactors Batch cultivation of Azadirachta indica hairy roots was ...

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Statistical medium optimisation for enhanced azadirachtin ...

View Statistical medium optimisation for enhanced azadirachtin production in hairy root culture of Azadir from BIOTECH 15BT201 at SRM University. In Vitro Cell.Dev.Biol.—Plant (2012) 48:73–84 DOI

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Enhanced production of azadirachtin by hairy root cultures ...

2007-2-1  Hairy roots were cultured in 70 ml of liquid media in 250 ml Erlenmeyer flasks and kept in gyratory shaker at 60 rpm.When three different media like ON, B5 and MS with 3% sucrose were tested, it was found that ON was the best medium for the growth of hairy root cultures and azadirachtin production followed by B5 and MS, respectively ().The roots grown in ON medium produced 0.0166%

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*PUBLICATIONS*

2018-6-13  Smita Srivastava, "In vitro azadirachtin (biopesticide) production by hairy root cultivation of Azadirachta indica in a modified bubble column reactor", Invited paper presentation under ISCA Young Scientists Award Programme for 2011-12 in

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Statistical medium optimization for enhanced

tages, in the present study in vitro azadirachtin production by hairy roots of A. indica was investigated as an alternative means for continuous production of azadirachtin, with the possibility of increasing azadirachtin yield by appropriate root line selection and manipulation of the culture environment.

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Azadirachtin technical mixture, analytical standard ...

Azadirachtin production by hairy root cultivation of Azadirachta indica in a modified stirred tank reactor. Smita Srivastava et al. Bioprocess and biosystems engineering, 35(9), 1549-1553 (2012-05-15)

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Induction of hairy root cultures of Azadirachta indica A ...

2002-11-1  Read "Induction of hairy root cultures of Azadirachta indica A. Juss. and their production of azadirachtin and other important insect bioactive metabolites, Plant Cell Reports" on DeepDyve, the largest online rental service for scholarly research with thousands of

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Soybean hairy roots produced in vitro by Agrobacterium ...

2018-4-1  1. Introduction. Agrobacterium rhizogenes contains a root-inducing (Ri) plasmid that contains root locus (rol) genes in the T-DNA region, including rolA, rolB, rolC, and rolD, and is able to induce hairy roots from the wounded surface of explants after infection .The hairy roots can be maintained in culture or hosted in composite plants with untransformed aerial tissue.

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PLANT CELL CULTIVATION OF A INDICA IN BIOREACTOR

A pre-optimized computer simulated continuous cultivation strategy with cell retention was experimentally implemented which featured high biomass and azadirachtin production from A indica cells in the bioreactor. The experimental verification resulted in a biomass accumulation of 61.4 g/L and azadirachtin production of 751.9 mg/L with 14% ...

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Production of biopesticide azadirachtin using plant cell ...

2017-4-17  Production of biopesticide azadirachtin using plant cell and hairy root cultures ... the inconsistent content and purity of the metabolites in whole plant makes it imperative to tap the potential of in vitro plant tissue ... This review gives a summarized literature of the applied research and achievements in plant cell/hairy cultures of A ...

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Induction of hairy root cultures of Azadirachta indica A ...

T1 - Induction of hairy root cultures of Azadirachta indica A. Juss. and their production of azadirachtin and other important insect bioactive metabolites. AU - Allan, Eunice Jessie. AU - Eeswara, J. P. AU - Jarvis, A. P. AU - Mordue, Anne Jennifer. AU - Morgan, E. D. AU - Stuchbury, Trevor. PY - 2002. Y1 - 2002

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Production of azadirachtin from plant tissue culture ...

With increasing awareness towards environment-friendly and non-toxic pesticide azadirachtin obtained from neem tree (Azadirachta indica) is gaining more and more importance. Its broad-spectrum activity, peculiar mode of action. eco-friendly and non-toxic action towards beneficial organisms has offered many advantages over chemical pesticides. All currently use commercial formulations based on ...

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Azadirachtin biosynthesis induction in Azadirachta indica ...

A. Juss. and analysis for in vitro azadirachtin production. In Vitro Cell Dev Biol Plant. 2003; 39(3): 304-310. [ Links ] Allan EJ, Eeswara JP, Jarvis AP, Mordue AJ, Morgan ED, Stuchbury T. Induction of hairy root cultures of Azadirachta indica A. Juss. and their production of azadirachtin and other important insect bioactive metabolites.

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Cannabinoids Production by Hairy Root Cultures of

2015-7-31  cluding illegality of cultivation and breeding of Cannabis [14], variability of active components [15]. To address these issues, in vitro tissue culture techniques have been exploited to obtain a standardized method for secondary metabolite production [16] [17]. Hairy root cultures are a promising source for production of valuable in vitro

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Kirenol production in hairy root culture of Siegesbeckea ...

Following 49 days of cultivation, time course of kirenol production in different hairy root culture media was achieved. The different hairy root clones showed variation in kirenol contents, with the white medium culture having the lowest and MS medium culture having the highest kirenol content [Figure 5c].

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Agrobacterium rhizogenes mediated hairy root induction

2017-8-29  Effect of infection and co‑cultivation periods on hairy root induction Leaves from in vitro grown microshoots were taken and cut into small pieces (approxi- mately 5 × 5 mm size), dipped in 48 h old culture of A. rhizogenes (MTCC 532 and/or

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Influence of acetosyringone concentration on induction

2018-12-7  Growth of hairy root on carrot discs. numerous lateral roots (Figure 4.), which grew both on the surface and penetrated into the media, exhibiting the Figure 5. Negative geotropism of hairy root . negative geotropic growth habit (Figure 5). Some carrot discs were observed without any hairy root

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Hairy Root Culture: History, Formulation and Application

2021-8-12  Scaling up of hairy roots in novel bioreactors can provide the best conditions for optimum growth and secondary metabolite production than in native root culture. Hairy root morphology is quite plastic so the bio­reactor design for root culture is a balancing act between plant tissues and surrounding control­led environment.

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Protocol: high-efficiency in-planta Agrobacterium-mediated ...

2018-2-23  High efficiency protocol for the generation of composite plants in tea. The use of ex vitro composite plants through A. rhizogenes—mediated transformation has been widely researched in various plant species [36,37,38].We have optimized the hairy root induction protocol in tea (Fig. 1; Table 2) that reliably generates high efficiency transformed roots suitable for root biology studies.

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